Our previous study demonstrated that germinated garlic extract and its hexane soluble fraction had antioxidant and neuroprotective effects. However, it is not known whether germinated garlic extract inhibit the invasion of cancer cells. The purpose of this study was to assess the effect of germinated garlic extracts on the invasion of human hepatoma cells (HepG2) by 12-O-tetradecanoylphorbol 13-acetate (TPA). Effect of germinated garlic extracts on cancer cell invasion was evaluated using wound healing assay. The effects of germinated garlic extract on the expression of matrix metalloproteinase (MMP)-2 and 9, mitogen-activated protein kinases (MAPKs), AKT, and nuclear factor-κB (NF-κB), were investigated with gelatin zymography and western blot analysis. Germinated garlic extract significantly suppressed TPA-induced cell invasion in sprouting period dependent manner, which was mediated by suppression of TPA-induced MMP-2, 9 expressions and NF-κB signaling pathway. Taken together, our results suggest that germinated garlic extract has potential to inhibit the metastasis of human hepatoma cells by inhibiting the expression of MMP-9 through inhibiting NF-κB signaling pathway.